應用編號(Application ID):PR0019
樣品分類(Sample Category):醫藥(Pharmaceutical)
分離模式(Separation Mode):反相(Reversed phase chromatography)
產品系列(Product Line):Sapphire
方法(Method):
Column: |
Sapphire-C18, 5 µm, 100 Å, 4.6 x 250 mm |
|
Mobile phase: |
A: H2O B: ACN |
|
Time(min): |
0 6 10
40 50 100 |
|
Flow rate: |
0.8 mL/min |
|
Temperature: |
Room temperature |
|
Detection: |
UV 242 nm |
|
Injection volume: |
10 µL |
|
Sample: |
1. 6β-OH R.S |
4. Hydrocortisone (氫化可的松) |
0.033 mg/mL each in CH3OH |