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Column:

GP-Phenyl, 5 µm, 120 Å, 4.6 x 250 mm

Mobile phase:


Time(min):
B%:

A: 0.064% Ammonium Formate, Adjusted to pH 3.5 with Formic Acid
B: Acetonitrile
0  2.5  10  15  17  18  40
5  5    12  30  30  5   5

Flow rate:

1.0 mL/min

Temperature:

25 ℃

Detection:

UV 270 nm

Injection volume:

4 µL

Sample:

3.0 mg/mL Cefquinome （頭孢喹諾） in mobile phase A