應用編號(Application ID):
樣品分類(Sample Category):生物化學(Biochemistry)
分離模式(Separation Mode):反相(Reversed phase chromatography)
產品系列(Product Line):Sapphire
方法(Method):
Column: |
Sapphire-C18 ( 5 µm, 100 Å, 4.6 x 250 mm ) |
Mobile phase: |
Citric acid ( dissolve 9.6 g of citric acid monohydrate in water to make 1000 mL ) : Methanol = 67 : 33 ( v/v ) |
Flow rate: |
1mL/min |
Temperature: |
40 ℃ |
Detection: |
UV 254 nm |
Injection volume: |
20 µL |
Sample: |
test sample ( 6mg/mL ) |