應用編號(Application ID):
樣品分類(Sample Category):生物化學(Biochemistry)
分離模式(Separation Mode):反相(Reversed phase chromatography)
產品系列(Product Line):Bio
方法(Method):
Column: |
Bio-C18 ( 5 µm, 200 Å, 4.6 x 250 mm ) |
Mobile phase: |
A: 0.1%TFA in H2O, B: 0.08%TFA in ACN |
Flow rate: |
1.0 mL/min |
Temperature: |
40℃ |
Detection: |
UV 214 nm |
Injection volume: |
20 µL |
sample: |
HSA enzymed by tryspin ( 0.85 mg/mL ) |